Fig. 7.

Relative quantification of message for connexin 43 (Cx43) and Cx40 by quantitative RT-PCR (qRT-PCR). Values are presented as means ± SD. RNA was prepared from duplicate samples extracted from bovine kidney and brain and from a single harvest of bovine ciliary epithelial cells. Each value was averaged from four real-time qRT-PCR measurements. Message detected in ciliary epithelial cells for Cx43 was two orders of magnitude larger than that for Cx40. Message for the widely distributed Cx43 was also detected in bovine kidney and brain. However, as expected, Cx40 was substantially expressed in kidney, but not in brain.