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. 2009 Nov 18;298(4):C831–C846. doi: 10.1152/ajpcell.00032.2009

Fig. 1.

Fig. 1.

Endogenous fibrocystin-1 in human autosomal recessive polycystic kidney disease (ARPKD) cells by comparison with normal human fetal collecting tubule (HFCT) cells. A: Western immunoblot analysis of fibrocystin-1 in age-matched normal HFCT and ARPKD epithelial cells. Confluent steady-state cells, 60 μg of cell lysate per lane. Full-length fibrocystin-1 is clearly detectable at >460 kDa in HFCT but barely detectable neither in ARPKD nor in PKHD1 siRNA-treated HFCT cells. Fibrocystin-1 1:500, actin 1:10,000. B: densitometry analysis of levels in HFCT and ARPKD after normalization to actin. Fibrocystin-1 expression ratio: HFCT/ARPKD 7:1. siRNA, small interfering RNA.