Fig. 7.

Time- and attachment dependent distribution of actin cytoskeleton in ARPKD cells by comparison to normal HFCT cells. Rhodamine-phalloidin fluorescent labeling of filamentous actin 30 min (A, B, G, H), 4 h (C, D, I, J), and 24 h (E, F, K, L) after attachment; 2,000 cells were plated per collagen I-coated well. Note the thin cortical actin staining in HFCT cells after 4 h of attachment (C and I); complex actin organization in ARPKD cells after 30 min (B and H) and 4 h of attachment (D and J). Filopodial projections are also seen in ARPKD epithelia (H, arrowheads); n = 10.