Fig. 3.

Identification of transcription factors binding to AP-1 elements within the arginase I promoter. A: electrophoretic mobility shift assay (EMSA) analysis was performed with nuclear extracts from RAECs treated with control and thrombin (30 U/ml) for 30 min. A 20-fold excess unlabeled probe was used to distinguish specific from nonspecific protein-DNA complex formation. Nuclear extracts from RAECs treated with thrombin formed a specific complex with oligonucleotides containing the wild-type AP-1 site. B: supershift assay was performed with nuclear extracts from RAECs treated with thrombin in the absence and presence of c-Jun and ATF-2 specific antibodies. Control IgG was used as a negative control. Thrombin receptor agonist peptide (TRAP) was used as a positive control. ATF-2, activating transcription factor 2. Results shown are representative of three independent experiments.