Fig. 4.

Chromatin immunoprecipitation (ChIP) assays demonstrate specific binding of c-Jun and ATF-2 to the −3,157 bp AP-1 site in response to thrombin. Assays were performed using anti-c-Jun, anti-ATF-2, and control antibodies, with RAECs treated with control or thrombin for 30 min. Two distinct sets of primers spanning different regions of arginase promoter were used to amplify the immunoprecipitated (IP) DNA. Top: amplified DNA, using primers spanning from −3,296 to −3,111 bp, containing the AP-1 site. Bottom: amplified DNA, using primers spanning from −2,340 to −2,157 bp as a negative control. The PCR products of both input and ChIP samples were loaded as indicated and analyzed using a 2% agarose gel. Results shown are representative of three independent experiments.