Table 2.

Effects of NO on normalized single-channel open and closed properties of recombinant Kir6.2/SUR1 channels in intact SH-SY5Y cells in the presence and absence of 5-HD and ROS scavengers

Properties	NOC-18	+5-HD	+MPG	+Catalase
Open probability	4.23 ± 0.79†	1.89 ± 0.26†	1.08 ± 0.21	1.00 ± 0.15
Opening frequency	3.52 ± 0.59†	1.62 ± 0.22*	0.97 ± 0.16	0.96 ± 0.11
Mean open duration	1.18 ± 0.05†	1.13 ± 0.09	1.05 ± 0.10	1.03 ± 0.07
Mean closed duration	0.37 ± 0.05‡	0.94 ± 0.45	1.65 ± 0.68	1.21 ± 0.19
Number of patches	7	10	8	9

Single-channel recordings of Kir6.2/SUR1 channels expressed in transfected SH-SY5Y cells were performed in the cell-attached patch configuration at −60 mV in symmetrical 140 mM K⁺ solutions. DETA NONOate (NOC-18) alone (300 μM) or NOC-18 (300 μM) in the presence of 5-HD (100 μM), MPG (500 μM), or catalase (500 U/ml) was applied by bath perfusion. Single-channel properties were obtained and normalized as described in Table 1 (control taken as 1). Data are means ± SE.

^* P < 0.05;

^† P < 0.01;

^‡ P < 0.0001 (two-tailed, one-sample t-tests).