Fig. 1.

Depletion of ADP-ribosylation factor-6 (Arf6) inhibits isoproterenol (Iso)-stimulated lipolysis in adipocytes. 3T3-L1 adipocytes were treated with a negative control small interfering RNA (siRNA, NC) or an siRNA-recognizing Arf6. A: 2 days posttransfection, whole cell lysates were prepared and analyzed by immunoblotting (IB) using antibodies as indicated. B: cells were starved and stimulated with 10 μM Iso for 1 h, and glycerol release was assayed and normalized to total protein. *P < 0.05. The data represent means ± SE of four independent experiments. C: cells were starved and stimulated with 10 μM Iso for 10 or 30 min, and glycerol release was assayed and normalized to total protein. *P < 0.05.