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. 2010 Jan 7;298(4):C921–C928. doi: 10.1152/ajpcell.00541.2009

Fig. 2.

Fig. 2.

Inhibition of dynamin, but not depletion of cholesterol, impairs Iso-stimulated lipolysis in adipocytes. A: 3T3-L1 adipocytes were starved and treated with DMSO (control, CTL) or dynasore (80 μM) for 20 min before stimulation with 10 μM Iso for 1 h, and the glycerol release was assayed and normalized to total protein. B: 3T3-L1 adipocytes were starved and treated with DMSO (CTL) or dynasore (80 μM) for 20 min before stimulation with 10 μM Iso for 10 or 30 min, and the glycerol release was assayed and normalized to total protein. C: 3T3-L1 adipocytes were starved and treated with 10 mM methyl-β-cyclodextrin (MCD) for 1 h before stimulation with 10 μM Iso for 1 h and the glycerol release was assayed. *P < 0.05. The data represent means ± SE of three independent experiments.