Fig. 5.

Arf6 depletion does not alter insulin signaling. A: 3T3-L1 adipocytes were starved and treated with 10 nM insulin (Ins) for 15 min before stimulation with 10 μM Iso for 1 h, and the glycerol release was assayed and normalized to total protein. *P < 0.05. The data represent means ± SE of three independent experiments. B: 3T3-L1 adipocytes were treated with negative control siRNA (NC) or an siRNA recognizing Arf6. Cells were starved and stimulated with Ins at indicated concentrations for 10 min. The whole cell lysates were prepared and subjected to immunoblot (IB) analysis utilizing antibodies recognizing pAkt (Ser473), total Akt (tAkt), pMAPK, and total MAPK (tMAPK). Results are representative of three independent experiments.