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. Author manuscript; available in PMC: 2010 Apr 12.
Published in final edited form as: Gastroenterology. 2008 Jul 31;135(5):1687–1697. doi: 10.1053/j.gastro.2008.07.026

Figure 3. shRNA-directed knock-down of endogenous Mist1 leads to decreased p21CIP1/WAF1 levels and increased proliferation.

Figure 3

(A) Immunoblots of AR42J-ControlRNAi and AR42J-Mist1RNAi cell lines showing that "knock-down" of endogenous Mist1 transcripts results in decreased p21CIP1/WAF1 levels. (B) Growth analysis of AR42J-ControlRNAi and AR42J-Mist1RNAi cells. (C) Immunofluorescence of AR42J-Mist1RNAi cells infected with pBrit or pBrit-mMist1ER virus. 90% of pBrit-Mist1ER cells were strongly or weakly (closed arrows) positive for Mist1ER while a small number of cells remained Mist1ER negative (open arrows). (D) Growth analysis of AR42J-Mist1RNAi cells infected with pBrit or pBrit-mMist1ER virus. Each time point corresponds to the average of 3 independent experiments (± s.d.).