Fig. 1.

Interferon (IFN)-γ induces intercellular adhesion molecule (ICAM)-1 expression in cultured human cholangiocyte. A and B: dose-dependent expression of ICAM-1 at the message and the protein levels in H69 cells following IFN-γ stimulation. H69 cells were exposed to the culture medium with various doses of IFN-γ (0, 0.1, 1.0, 10, and 25 ng/ml) followed by real-time PCR (after incubation for 8 h) or Western blotting analysis for ICAM-1 (after incubation for 24 h). C and D: time-dependent expression of ICAM-1 expression in H69 cells induced by IFN-γ. Cells were exposed to IFN-γ (10 ng/ml) followed by real-time PCR (incubation for up to 24 h) or Western blot (incubation for up to 48 h). E and F: IFN-γ-induced expression of ICAM-1 in human intrahepatic biliary epithelial (HIBEpiC) cells. HIBEpiC cells were exposed to culture medium with or without IFN-γ (10 ng/ml) followed by real-time PCR (incubation for 8 h) or Western blot (incubation for 24 h). Bars represent the means ± SD from 3 independent experiments. *P < 0.05 t-test vs. the non-IFN-γ-stimulated control.