Fig. 4.

Functional inhibition of miR-221 increases ICAM-1 protein expression but does not alter ICAM-1 mRNA level. A: anti-miR-221 increased ICAM-1 protein expression in cholangiocytes. H69 cells were treated with various doses of anti-miR-221 for 24 h followed by Western blot for ICAM-1. B: anti-miR-221 did not alter ICAM-1 expression at the message level in cholangiocytes. H69 cells were treated with various doses of anti-miR-221 for 24 h followed by real-time PCR for ICAM-1. Bars represent the means ± SD from 3 independent experiments. Anti-miR-Ctrl, nonspecific anti-miR control; *P < 0.05 t-test vs. non-anti-miR-221-treated cells.