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. 2010 Jan 28;298(4):G542–G550. doi: 10.1152/ajpgi.00490.2009

Fig. 5.

Fig. 5.

IFN-γ decreases miR-221 expression in a signal transducer and activator of transcription (STAT)1-dependent manner. A and B: IFN-γ stimulation decreased miR-221 expression in cholangiocytes. H69 and HIBEpiC cells were exposed to IFN-γ (10 ng/ml) for 8 h or 12 h followed by Northern blot for miR-221 (in H69 cells in A) and real-time PCR (in H69 and HIBEpiC cells in B). RNU6B (U6) was used as the control. C: knockdown of STAT1 blocked IFN-γ-induced decrease of miR-221 expression. H69 cells were treated with either the STAT1 siRNA or the scrambled control siRNA for 48 h and then exposed to IFN-γ (10 ng/ml) for additional 8 h. Total RNA was isolated, and the expression of miR-221 was quantified by real-time PCR. Data are representative of 3 independent experiments. Bars represent the means ± SD from 3 independent experiments. *P < 0.05 t-test vs. non-IFN-γ-stimulated cells in B and C; #P < 0.05 t-test vs. the scrambled siRNA control in C.