Fig. 1.

RNA and protein expression of adenylyl cyclases (ACs) in acutely isolated mouse inner medullary collecting duct (IMCD). A: PCR of mRNA of the 9 membrane-associated AC isoforms from a representative gel (n = 5 total experiments) in IMCD and brain (the latter as a positive control). Note that the dashed line in the brain PCR samples indicates where 2 different PCR gels are shown; they are placed adjacent to one another to facilitate viewing. B: representative Western analysis for AC3, AC4, AC6, AC7, and AC9 in IMCD (n = 4 total experiments) and AC7 and AC9 in brain. The antibody for AC6 also recognizes AC5; since no AC5 mRNA was detected in acutely isolated mouse IMCD, it is assumed that the band represents AC6. Western blot analysis was not done for AC isoforms lacking mRNA in the IMCD. The approximate expected sizes of AC3, AC4, AC6, AC7, and AC9 based on amino acid sequence without including possible posttranslational modification are 137, 129, 140, 132, and 162 kDa, respectively. Size indications in kDa are shown.