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Cellular cAMP accumulation in acutely isolated mouse IMCD. Tubules were preincubated with 1 mM IBMX for 30 min and then incubated with medium alone, 25 μM W-7, 10 μM KN-93, or 5 μM thapsigargin (Thaps) for 30 min, followed by addition of 1 nM AVP (A) or 1 μM forskolin (B) for 5 min (n = 6 each data point). *P < 0.001 vs. AVP or forskolin alone; **P < 0.01 vs. forskolin alone and vs. W-7 + forskolin.
