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Cellular cAMP accumulation in acutely isolated mouse IMCD. Cells were preincubated with 1 mM IBMX for 30 min and then incubated with medium alone, 10 μM H-7, or 500 nM calphostin C for 30 min, followed by addition of 1 nM AVP for 5 min (n = 12 each data point). *P < 0.05 vs. AVP alone.
