Table 2.
PCR signal intensity in mouse collecting duct for AC isoforms
| Adenylyl Cyclase Isoform |
|||||||||
|---|---|---|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | |
| M-1 cell line | − | + | + | + | − | + | ± | − | − |
| mIMCD3 cell line | − | + | + | + | + | + | ± | − | − |
| mpkCCD cell line | − | − | + | + | − | + | ± | − | ± |
| IMCD primary culture | − | ± | + | + | − | + | ± | − | ± |
| IMCD acutely isolated | − | ± | + | + | − | + | ± | − | ± |
| OMCD microdissected | − | ± | + | + | + | + | ± | − | − |
| CCD microdissected | − | ± | + | + | + | + | ± | − | − |
−, None detected; ±, inconsistent or weak signal; +, strong consistent signal; n =5 for each data point. IMCD, inner medullary collecting duct; OMCD, outer medullary collecting duct; CCD, cortical collecting duct.