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Isoflurane (2.5% for 14 h) increases caveolin-1 and caveolin-2 protein in the buoyant fractions of SK1-HK-2 cells. Confluent SK1-HK-2 cells grown in 10-cm plate were processed for each experiment. Control cells were treated with carrier gas (95% air-5% CO2) for 14 h. Caveolin immunoblotting was used to detect the caveolae microdomain fraction. Caveolin-1 immunoblotting showed 2 isoforms. Similar results obtained from 4 independent experiments.
