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A: treatment with PC/PS mixture (100 μM) or TGF-β1 (1 ng/ml) for 14 h increases S1P formation in the buoyant fractions of SK1-HK-2 cells (n = 6). *P < 0.05 vs. carrier gas. B: pretreatment with neutralizing TGF-β1 antibody (1 μg/ml) prevents the isoflurane (2.5% for 14 h)-induced increase in S1P formation in the buoyant fractions (n = 6). Confluent SK1-HK-2 cells grown in 10-cm plates were processed for each experiment. *P < 0.05 vs. carrier gas.
