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HPLC analysis for S1P levels in caveolae fractions isolated from murine renal cortices. TGF-β1 wild-type (+/+) or heterozygous (+/−) mice were either anesthetized with pentobarbital sodium (PB) or with isoflurane (ISO; 1.2%) for 4 h. Representative data from 3 independent experiments. By introducing C17-S1P as an internal standard (Int Std), we can quantify the amount of S1P present in the original sample.
