Fig. 2.

Metabolite intensities of top 50 most influential metabolites for discriminating between genotypes as identified through partial least squares (PLS) regression and linear discriminant analysis (LDA). The bar at the top of the figure indicates the genotype of each individual [blue: wild-type (wt); red: jck]. Blue hues indicate higher intensities, and red hues indicate lower intensities. At 26 (A) and 45 days (B), metabolite intensities clustered according to genotype, suggesting metabolic differences between genotypes and similarities within genotypes. At 64 days (C), clustering according to genotype was less evident. Clusters were identified through hierarchical clustering (30) using Euclidean distances between body weight-adjusted intensity measurements from each mouse.