Fig. 3.

PLS graphs show separation between jck and wild-type mice. Scores of each mouse on first and second latent components are identified through PLS regression using body weight-adjusted intensity measurements at 26 (A), 45 (C), and 64 days (E) and intensity measurements that were not body weight adjusted (B: 26 days; D: 45 days; F: 64 days). Each data point represents 1 animal. Cystic (jck; ●) and wild-type (□) phenotypes were separable by urinary metabolomic analysis at 26 and 45 days regardless of adjustment for body weight. At 64 days, cystic and wild-type phenotypes were not separable with PLS using body weight-adjusted intensities but could be separated when intensities were not body weight adjusted.