Fig. 6.

ENaC coimmunoprecipitates with NOX2 catalytic domain. Western blot (WB) analysis of immunoprecipitated (IP) protein, derived from rat primary alveolar epithelial cells, using either goat anti-α-ENaC subunit antibody (lane 1) or rabbit anti-NOX2 antibody (lane 2; positive signal control). Detection of NOX2 in IP studies was done so using standard Western blot protocols and rabbit anti-NOX2 primary antibody. The typical glycosylation smear for NOX2 [which runs between 90 and 65 kDa (20)] was observed in both lane 1 and 2.