Skip to main content
. Author manuscript; available in PMC: 2011 Apr 1.
Published in final edited form as: Curr Opin Microbiol. 2010 Mar 10;13(2):168–176. doi: 10.1016/j.mib.2010.01.013

Figure 3.

Figure 3

EnvZc binding to OmpR using fluorescence anisotropy. The binding reactions contained 40 nM fluorescein-labeled OmpR. Closed triangles show OmpR binding to EnvZc with a Kd of 519 nM. The average Kd for EnvZc binding to OmpR from 10 independent experiments was 425 ± 127 nM. In the presence of specific DNA, EnvZc still binds OmpR, with a Kd of 568 nM (open triangles). The average Kd for EnvZc binding to OmpR C1-C2-C3 from six separate curves is 385 ± 162 nM. Reprinted with permission from [29].