Figure 1.

Glycogen content (referred as percentage of glycogen accumulated by WT cells) of glycogen-excess and glycogen-deficient clones of the ASKA library. Averaged glycogen content in WT cells was 45 nmol glucose mg protein⁻¹. Cells were grown at 37°C with rapid gyratory shaking in liquid Kornberg medium (1.1% K₂HPO₄, 0.85% KH₂PO₄, 0.6% yeast extract) supplemented with 50 mM glucose. Because some yeast extracts are deficient in Mg²⁺,³ and because Mg²⁺ is a major determinant of cell metabolic and energetic status and of expression of genes affecting glycogen metabolism,^91,92 the Kornberg medium was also supplemented with 1 mM MgCl₂.