Fig. 3.

BK-induced activation of Pak1 associated with dephosphorylation of cardiac troponin I (cTnI). A: inhibition of phosphorylation of cTnI by constitutively active Pak1 and by BK, as detected by [³²P] incorporation: myocytes infected with adenovirus Pak1 (AdPak1; lane 1), myocytes infected with AdLacZ (control; lane 2), myocytes infected with AdLacZ and treated with 100 nM isoproterenol (Iso; lane 3), and myocytes infected with AdLacZ and treated with BK at 10 μM (lane 4). B: histogram showing dephosphorylation of cTnI induced by BK at 1 and 10 μM (n = 3). Signal intensity is given in arbitrary units. Results shown are measurements from 4 independent experiments. *P < 0.05 compared with control without exposure to BK.