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. 2010 Feb 12;298(4):H1283–H1289. doi: 10.1152/ajpheart.01070.2009

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Fig. 5. — Demonstration of protein phosphate 2A (PP2A) association with both Pak1 and PLB by immunoprecipitation (IP). A: PP2A association with Pak1 when Pak1 antibody was used in IP (lane 1), negative control with the same experimental conditions except without the primary antibody (lane 2), and Pak1 association with PP2A when PP2Ac antibody was used in IP. B: Western blotting showing the presence of both PP2Ac and PLB in IP products using a PLB antibody. A control with no PLB or PP2A antibody showed no staining.