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. 2010 Mar 16;11:37. doi: 10.1186/1471-2202-11-37

Figure 1.

Figure 1

NMMII is localized pre- and postsynaptically at the NMJ. α-NMMII (red) was found to colocalize with the neural marker α-HRP (green). α-NMMII staining is observed which did not colocalize with α-HRP (merge) suggesting NMMII is also present postsynaptically (top row). The postsynaptic marker α-Dlg (green) is observed to colocalize (merge) with α-NMMII (red) suggesting NMMII is present postsynaptically (second row). To confirm presynaptic expression of NMMII, UASzipRNAi was expressed postsynaptically using 24BGal4 (bottom row). This eliminated postsynaptic expression of NMMII. Immunocytochemistry revealed that while postsynaptic staining of NMMII had been eliminated, NMMII was still present presynaptically as seen with the colocalization with α-HRP (merge). No postsynaptic NMMII is visible in the merged image. The top and bottom rows are shown as confocal stacks, while the middle two rows are shown as single confocal slices.