Identification of tauro-7-epicholate and tauro-3α,6(α/β),7α,12α-tetrol. A: Chromatograms of synthetic tauro-7-epicholate (upper panel) and mouse urine (lower panel). Both ion chromatograms were extracted in the 50 ppm mass range of tauro-7-epicholate (514.2839 m/z) in the negative ion mode. B: Chromatograms of synthetic tauro-3α,6(α/β),7α,12α-tetrol (upper panel) and mouse urine (lower panel). Both ion chromatograms were extracted in the 50 ppm mass range of tauro-3α,6(α/β),7α,12α-tetrol (530.2788 m/z) in negative ion mode. The values of retention time for several peaks are listed above their respective peak. In mouse urine, several peaks confirmed with authentic compounds are labeled: tauro-7-epicholate (a), tauro-β-muricholate (b), taurocholate (c), and tauro-3α,6(α/β),7α,12α-tetrol (d).