TABLE 2.

Distribution of DChol in cholesterol-rich and -poor microdomains isolated from L-cell fibroblast plasma membranes by affinity chromatography

	DChol (nmol/mg protein)		Ratio in Cholesterol-Rich/Cholesterol-Poor Microdomains	% Chol Replaced by DChol
Incubation with DChol (h)	Cholesterol-Rich Microdomains	Cholesterol-Poor Microdomains		Cholesterol-Rich Microdomains	Cholesterol-Poor Microdomains
0.25	2.06 ± 0.69	0.98 ± 0.58	2.10 ± 0.84	0.21 ± 0.07	0.25 ± 0.15
1	4.52 ± 0.90	4.53 ± 0.18	0.99 ± 0.16	0.46 ± 0.09	1.16 ± 0.05
24	7.10 ± 0.65	9.37 ± 0.25	0.76 ± 0.04	0.72 ± 0.05	2.4 ± 0.06

L-cells were incubated with DChol-MβCD complex (20 μg/ml medium) for the indicated times as described in Methods. Cells were then fractionated to isolate plasma membranes, which were in turn resolved into cholesterol-rich and -poor microdomain enriched fractions by affinity chromatography, lipids were extracted, and DChol and cholesterol were quantitated as described in Methods. Values represent mean ± SEM of two experiments.