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Single bovine embryo MALDI-MS/MS for the ions of: (A) m/z 810.6 [PC (38:4) + H]+ or [PC (36:1) + Na]+ and (B) m/z 725.5 [SM (16:0) + Na]+. Both spectra display characteristic NL of 59 Da (trimethylamine) and of 124 Da from the loss of the cyclophosphane ring. Also, the sodiated cyclophosphane (m/z 147.0) and the choline polar head group (m/z 184.0) ion can be observed.
