Fig. 1.

Caveolin-3 modulates transmembrane FA movement. A: Parental cells were transiently transfected with caveolin-3 (or not) as outlined in “Experimental Procedures.” Whole-cell extracts were prepared and subjected to SDS-PAGE as was a rat muscle cell membrane preparation. B: The proteins were transferred and subjected to Western blotting, and detection was carried out with HRP-conjugated secondary antibody and chemiluminescence. A representative trace for BCECF fluorescence is shown in both parental (P) and high caveolin-1 expressing (B8) cells. C: The data are presented as the average of triplicates from four independent experiments comparing fluorescence values after 600 s for caveolin-3 transfected (P/cav3) and untransfected cells (P) as well as B8 cells (* = P ≤ 0.003, P/Cav3 versus P). BCECF, 2′7′-bis-(2-carboxyethyl)-5-(and6)-carboxyfluorescein.