Fig. 4.

Caveolin expression enhances cellular FA accumulation. A: After 48 h with 80 μM oleic acid, cells were fixed and stained with Oil Red O as described in “Experimental Procedures.” B: Triglyceride accumulation was determined and normalized to total cellular protein. The results are from 8 cell preparations with P ≤ 0.001 for high caveolin-1 expressing cells (B8) and caveolin-3 transfected (P/ Cav3) versus untransfected (P) cells at 48 h. C: Lipid droplets were isolated as described by Liu et al. (17). After SDS-PAGE and transfer, they were immuno­blotted for the indicated proteins as in Fig 1. ADRP, adipose differentiation-related protein; OA, oleic acid; TG, triglyceride.