Fig. 1.

LysoPC in oxLDL is not responsible for the generation of [Ca²⁺]_i oscillations. Calcium mobilization was visualized using fluo-4-AM and confocal fluorescence microscopy. After incubation with fluo-4-AM, BMDMs were washed, and media containing either nLDL (25 µg/ml), oxLDL (25 µg/ml), PC (10 µmol/l), or lysoPC (10 µmol/l) was added at time 0. Fluorescence values as a function of time were measured for every cell in the field, and cells positive for [Ca²⁺]_i oscillations are expressed as a fraction of total cells. ** P < 0.01 compared with cells treated with oxLDL.