Fig. 9.

PE adducts form to a greater extent than protein adducts in cells. IsoK was added for 2 h at increasing concentrations to HEK293 or HUVEC cells plated in six well plates and the amount of PE and protein adduct quantified by LC/MS/MS and normalized to amount of DNA in each well. A: Levels of PE and protein adduct in HEK293 cells incubated with 0-10 μM IsoK. B: Levels of PE and protein adduct in HUVEC cells incubated with 0-1 μM IsoK. IsoK, γ-ketoaldehyde isomer; PE, phosphatidylethanolamine.