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. 2010 Mar 31;10:30. doi: 10.1186/1472-6750-10-30

Table 4.

Continuous expression of p24 protein from several transgenic insect cell lines as determined by ELISA.

Cell line and passage number gag transgene presence (PCR) Gag expression:
pg p24/ml culture supernatant (1.5 × 106 cells)
pXLNeoGag passage 7 in Sf9 cells + 129.87 pg/ml
pXLNeoGag passage 13 in Sf9 cells + 129 pg/ml
pXLNeoGag passage 23 in Sf9 cells + 75.47 pg/ml
pXLNeoGag passage 93 in Sf9 cells + 606.6 pg/ml

The presence and active transcription of the gag transgene in the Sf9 cell line pXLNeoGag, was confirmed over several passages using PCR and p24 ELISA screening, respectively.