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RT-PCR analysis of Oct4. A) Expression of Oct4 (Primer set 1, 577 bp) in freshly isolated c-kit+ haematopoietic cells (T0) and following culture (T3--T10); EC 2102Ep cells (+ control); water (- control); normal hepatocytes (NL); diseased hepatocytes (DL); HepG2 cells; Hek293T cells (Hek); human mast cell line (HMC1). B) β-actin controls (245 bp). C) Expression of Oct4 (Primer set 2, 647 bp) in c-kit+ cells (T0-T10); EC 2102Ep (+ control); water (-).
