Table 3.
Identified proteins in HEAN and CHAR steers by 2D-gel and MS analysis
| Spota | Accessionb | Protein name | Scorec | Cd | Ne | MW(kDa/pI) | sequencef | Average Fold changeg |
|---|---|---|---|---|---|---|---|---|
| C7 | 114053019 | alpha-1-B glycoprotein [Bos taurus] | 255/56 | 17 | 7 | 54.1/5.29 | R. FPLGPVTSTTR.G R.LEGEDQFLEVAEAPEATQATFPVHR.A R.VLSPAGPEAQFELR.G R.ALWTGALTPGR.D R.CEAEVPDVSFLLLR.A R.SLLSELSDPVELR.V |
-3.7 |
| C12 | 2501351 | serotransferrin precursor [Bos taurus] | 176/55 | 7 | 3 | 79.9/6.75 | K.SVDDYQECYLAMVPSHAVVAR.T K.GEADAMSLDGGYLYIAGK.C K.FDEFFSAGCAPGSPR.N |
-2.8 |
| C13 | 3024627 | Alpha-soluble NSF attachment protein [Bos taurus] | 289/54 | 32 | 8 | 33.6/5.39 | K.NSQSFFSGLFGGSSK.I K.HDAATCFVDAGNAFK.K K.ADPQEAINCLMR.A K.VAGYAAQLEQYQK.A K.YEELFPAFSDSR.E K.LLEAHEEQNVDSYTEAVK.E R.LDQWLTTMLLR.I |
+2.7 |
| C27 | 119924886 | purine nucleoside phosphorylase [Bos taurus] | 423/56 | 39 | 10 | 32.4/6.25 | M.ENGFTYEDYQDTAK.W R.LTQAQTFDYSEIPNFPKSTVPGHAGR R.DHINLPGFSGENPLR.G R.FPAMSDAYDR.D R.NLGADAVGMSTVPEVIVAR.H R.VFGFSLITNK.V K.VIMDYESQGK.A K.ADHKEVLEAGK.Q |
-1.6 |
| C36 | 162287374 | polymerase I and transcript release factor isoform 2 [Bos taurus] | 311/57 | 20 | 9 | 43.4/5.49 | K.SDQVNGVLVLSLLDK.I K.IIGAVDQIQLTQAQLEER.Q R.QAEMEGAVQSIQGELSK.L K.KLEVNEAELLR.R K.LEVNEAELLR.R K.ATEMVEVGAEEEEGGAER.G |
+1.8 |
| C42 | 74 | annexin I [Bos taurus] | 600/57 | 45 | 20 | 39.2/6.44 | K.QAWFIENEEQEYIK.T K.GGPGSAVSPYPTFNPSSDVEALHK.A K.GVDEATIIEILTK.R K.KALLGHLEEVVLALLK.T K.ALLGHLEEVVLALLK.T K.TPAQFDAEELR.A K.GLGTDEDTLNEILASR.T R.SEELAVNDDLADSDAR.A K.GTDVNVFTTILTTR.S K.VLDLELK.G K.CATSQPMFFAEK.L K.LYGISLCQAILDETK.G |
+1.7 |
| H21 | 164420731 | Transaldolase 1 [Bos taurus] | 159/56 | 10 | 3 | 37.8/7.03 | K.LGGSQEEQITNAIDK.L K.LFVLFGAEILK.K K.SYEPQEDPGVK.S |
+1.7 |
| H29 | 27807289 78365297 |
Annexin [Bos taurus] glycerol-3-phosphate dehydrogenase 1 (soluble) [Bos taurus] |
494/56 370/56 |
28 29 |
11 10 |
38.8/6.92 38.2/6.42 |
R.DALNIETAIK.T K.GVDEVTIVNILTNR.S K.TPAQYDASELK.A R.TNQELQEINR.V K.DIVSDTSGDFR.K R.AEDGSVIDYELIDQDAR.D K.SYSPYDMLESIK.K K.SLYYYIQQDTK.G K.VCIVGSGNWGSAIAK.I R.VTMWVFEEDIGGR.K R.KLTEIINTQHENVK.Y K.LTEIINTQHENVK.Y K.ADTIGVSLIK.G K.LISEVIGER.L R.LGIPMSVLMGANIANEVADEK.F R.LGLMEMIAFAK.L K.FPLFTAVYK.V |
+1.5 |
| H30 | 194680257 | glucose-6-phosphate dehydrogenase [Bos taurus] | 270/56 | 16 | 7 | 62.9/6.9 | R.DGLLPEDTYIVGYAR.S R.NSYVAGQYDDTASYK.R R.DLQSSNQLSNHIASLFHEDQIYR.I K.KPGMFFNPEESELDLTYGNR.Y K.LPDAYER.L R.GPVEADELMK.R |
-1.5 |
| H32 | 62751863 | brain creatine kinase [Bos taurus] | 784/56 | 46 | 27 | 42.9/5.47 | R.FPAEDEFPDLSGHNNHMAK.V K.VLTPELYAELR.A R.GFCLPPHCSR.G K.LAVEALSSLDGDLAGR.Y K.SMTEAEQQQLIDDHFLFDKPVSPLLLASGMAR K.PVSPLLLASGMAR.D K.TFLVWINEEDHLR.V R.FCNGLTQIETLFK.S K.RGTGGVDTAAVGGVFDVSNADR.L R.LGFSEVELVQMVVDGVK.L K.LLIEMEQR.L R.LEQGQAIDDLMPAQK.- |
+1.5 |
| H41 | 221325670 115496400 |
malic enzyme 1, NADP(+)-dependent, cytosolic [Bos taurus] dihydropyrimidinase-like 2 [Bos taurus] |
618/56 356/56 |
26 16 |
16 6 |
63.9/5.86 62.6/5.95 |
R.DLAFTLEER.Q R.QQLNIHGLLPPSFISQDVQVLR.V R.LNSDFDR.Y R.YLLLMDLQDR.N K.VLMSDIEK.F R.GLFISIHDR.G R.GHISSVLNAWPEDVIK.A K.AIVVTDGER.I K.IWLVDSK.G K.AECTAEQCYK.L R.AIFASGSPFDPVTLPSGK.S K.IFLTTAEVIAQQVSDK.H K.TATVYPEPPNK.E K.QIGENLIVPGGVK.T R.DIGAIAQVHAENGDIIAEEQQR.I R.SITIANQTNCPLYITK.V K.DNFTLIPEGTNGTEER.M R.GSPLVVISQGK.I R.GLYDGPVCEVSVTPK.T |
+1.4 |
| H44 | 13096153 | Retinal Creatine Kinase [Bos taurus] | 784/56 | 46 | 27 | 42.8/5.48 | R.FPAEDEFPDLSGHNNHMAK.V K.VLTPELYAELR.A R.GFCLPPHCSR.G K.LAVEALSSLDGDLAGR.Y K.SMTEAEQQQLIDDHFLFDKPVSPLLLASGMAR.D K.PVSPLLLASGMAR.D K.TFLVWINEEDHLR.V R.FCNGLTQIETLFK.S K.RGTGGVDTAAVGGVFDVSNADR.L R.LGFSEVELVQMVVDGVK.L K.LLIEMEQR.L R.LEQGQAIDDLMPAQK.- |
+1.6 |
| H47 | 75832090 | isocitrate dehydrogenase 1 (NADP+), soluble | 1022/56 | 55 | 36 | 47.1/6.13 | K.IQGGSVVEMQGDEMTR.I K.CATITPDEK.R R.NILGGTVFR.E R.LVSGWVKPIIIGR.H R.ATDFVVPGPGK.V K.VEISYTPSDGSPK.T K.TVYLVHNFTESGGVAMGMYNQDK.S K.SIEDFAHSSFQMALSK.N R.FKDIFQEIYDK.Q K.SEFEAQNIWYEHR.L R.LIDDMVAQAMK.S K.SEGGFIWACK.N K.TVEAEAAHGTVTR.H K.GQETSTNPIASIFAWTR.G K.ALEEVCIETIEAGFMTK.D K.DLAACIK.G R.SDYLNTFEFMDK.L K.LGENLQLK.L |
+1.4 |
aSpot number as given on the 2-D gel image. C spot from CHAR crossbred, H spot from HEAN crossbred
bgi number from NCBI database of matched protein
cMascot score/Mascot threshold score for each identified protein.
dPercent sequence coverage (%)
eNumber of matched peptides
fThe sequence of matched peptides
gThe expression level (in fold change) in High BF tissues compared to Low BF tissues