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PCR amplification of the DNA recovered by immunoprecipitation from chromatin with an anti-CENP-A antibody. Samples were from Chr 19 (GM 10449): 1 and 4; Chr 5 (GM 10114): 2 and 5; and Chr 5 (GM11714): 3 and 6. Amplification was performed with oligoprimers specific for D19Z3 and D5Z2, shared with Chr 1 for both chromosomes: 1, 2 and 3. The locus common to chromosomes 5 and 19 only was amplified with the appropriate oligoprimers: 4, 5 and 6. M = 100 bp marker.
