Table 4.
ESTs expressed as His6-tagged proteins and screened with anti-saliva antiserum
| EST Cluster/singleton | Equivalent cluster on S1 datasheet | Template DNA | Size (KDa)* | Western-Blot | Best match to deducted orfs | |
|---|---|---|---|---|---|---|
| 1 | TAg-5-t | sgmgfb-contig_369 | GMsg-41d10 | 24 | positive | Tsetse TAg-5 |
| 2 | GMsg-7018 | sgmgfb-contig_4047 | GMsg-15f12 | 26.9 | positive | secreted salivary phospholipase A2, group XIIA |
| 3 | GMsg-8079 | sgmgfb-contig_5222 | GMsg-45 g06 | 35.45 | positive | salivary mucin |
| 4 | GMsg-7305 | sgmgfb-contig_1606 | GMsg-06 h03 | 28.8 | positive | Salivary expressed serine protease |
| 5 | GMsg-4676 | sgmgfb-contig_4626 | GMsg-116b04 | 18.6 | negative | conserved hypothetical protein |
| 6 | GMsg-4602 | sgmgfb-contig_16226 | GMsg-76e12 | 29 | negative | Synaptotagmin |
| 7 | GMsg-4579 | sgmgfb-contig_16256 | GMsg-77 g03 | 10 | negative | Serine proteinase inhibitor (KU family) |
| 8 | GMsg-7037 | sgmgfb-contig_5650 | GMsg-149 h01 | 47 | negative | Molecular chaperone (DnaJ superfamily) |
| 9 | GMsg-7847 | sgmgfb-contig_6058 | GMsg-164 h01 | 37 | negative | ATP synthase, subunit b |
| 10 | GMsg-8221 | sgmgfb-contig_2150 | GMsg-116b02 | 24 | negative | Unknow |
| 11 | GMsg-9862 | sgmgfb-contig_14095 | GMsg-123a01 | 19 | negative | Putative arsenite-translocating ATPase |
| 12 | GMsg-10713 | sgmgfb-contig_15840 | GMsg-58e12 | 26 | negative | Xaa-Pro aminopeptidase |
| 13 | GMsg-3464 | sgmgfb-contig_315 | GMsg-06 h09 | 21,5 | negative | Ferritin-1 heavy chain |
| 14 | GMsg-5226 | sgmgfb-contig_1969 | GMsg-58 g10 | 43 | negative | Peptidase A1 |
| 15 | GMsg-6756 | sgmgfb-contig_1231 | GMsg-169a09 | 32 | negative | Unknown |
| 16 | GMsg-6848 | sgmgfb-contig_984 | GMsg-94e07 | 18.6 | negative | Unknown |
| 17 | GMsg-8002 | sgmgfb-contig_699 | GMsg-102d06 | 35.5 | negative | Unknown |
| 18 | GMsg-5890 | sgmgfb-contig_290 | GMsg-148f11 | 22 | negative | Serpin |
| 19 | GMsg-8367 | sgmgfb-contig_930 | GMsg-115a09 | 58.7 | negative | Serine-type endopeptidase |
| 20 | GMsg-8399 | sgmgfb-contig_499 | GMsg-17 g08 | 56 | negative | disulfide isomerase |