Table 1.
Preparation of mock mixed infections
| Well # | P. falciparum | P. malariae | P. ovale | P. vivax |
|---|---|---|---|---|
| Concentration in Parasites/μL | ||||
| 1 | 400 | 40 | 400 | 40 |
| 2 | 40 | 40 | 400 | 40 |
| 3 | 40 | 40 | 40 | 40 |
| 4 | 40 | 40 | 4 | 40 |
| 5 | 4 | 40 | 4 | 40 |
| 6 | 4 | 40 | 4 | 4 |
| 7 | 40 | 400 | 40 | 40 |
| 8 | 4 | 400 | 4 | 40 |
| 9 | 400 | 40 | 400 | 400 |
| 10 | 40 | 40 | 4 | 400 |
| 11 | 40 | 4 | 4 | 40 |
| 12 | 400 | 40 | 40 | 400 |
| 13 (F) | 400 | - | - | - |
| 14 (M) | - | 400 | - | - |
| 15 (O) | - | - | 400 | - |
| 16 (V) | - | - | - | 400 |
| 17 (N) | - | - | - | - |
Mock mixed infections were prepared as shown. 2 ul of each species was mixed with equal volumes of the other species as shown. One microliter of each mixture was then used for the PCR assay.