Figure 1. CAR-mediated repression of cell death.

Approximately the same number (1×10⁵) of primary hepatocytes prepared from the livers of Car^+/+ and Car^−/− mice were plated on plastic dishes (24 wells). Cells were pretreated with DMSO or 250 nM TCPOBOP for 24 hours and were subsequently treated with or without TNFα (20 ng/ml) plus ActD (0.2 µg/ml) for 16 hours. Release of lactate hydrogenase (LDH) in culture media was determined as described in the Materials and Methods. Each value represents the mean +/− S.D as fold changes relative to DMSO without TNFα and ActD, which was independently reproduced 3 times. Symbol # indicates statistical significance between DMSO-pretreated cells and TCPOBOP-pretreated cells (p<0.05).