Figure 3. Deficiency in mouse P2Y₁₂ receptors diminishes thrombus formation on collagen and provokes disaggregation under flow.

Blood from P2Y₁₂ deficient (P2Y₁₂ ^−/−) or wild type (P2Y₁₂ ^+/+) mice was perfused over native type I collagen at 1000 s⁻¹ for 4 min in the presence of 10 µM ADP. (A) Quantitative effect of P2Y₁₂ deficiency on surface area coverage by platelets. (B) Average number of disaggregation events measured from a preformed aggregate during flow (n = 3). (C) Flow-cytometric evaluation of ADP-stimulated platelets with active α_IIbβ₃ (JON/A mAb) and of convulxin-stimulated platelets exposing P-selectin (anti-CD62 mAb) in whole blood. Experiments were performed as in Fig. 2. Data are means ± SE (n = 5–6), *p<0.05.