Figure 3. hycE gene expression in W3110.

RNA was isolated from anaerobic cultures grown to log phase at pH 5.5, 6, 6.5, and 7. Real-Time PCR was used to measure the mRNA levels of hycE, the large subunit of Hyd-3. Expression levels were normalized to the pH 7 control. Error bars represent SEM, n = 3 (RNA from independent cultures). The experiment was conducted twice.