Table 1. Effects of MVL-PLA2 on the variables of microtubule dynamic instability in living HMEC-1 cells.

Variables		Control	100 nM MVL-PLA2	% change	100 nM BPB-inactivated MVL-PLA2	% change
% of dynamic microtubules		58.5±4.0%	78.1±4.1%	**	76.7±7.4%	*
Rate (µm/min)	G	7.12±0.29	9.35±0.36	+31% ***	7.71±0.50	NS
	S	8.79±0.38	9.92±0.45	+13% **	12.54±0.83	+43% ***
Length (µm)	G	1.06±0.03	1.03±0.04	NS	1.34±0.07	+26% ***
	S	1.32±0.05	1.29±0.06	NS	1.81±0.13	+37% ***
Duration (min)	G	0.149±0.008	0.110±0.004	−26% ***	0.174±0.012	NS
	S	0.150±0.006	0.130±0.006	−13% *	0.145±0.008	NS
	P	0.315±0.015	0.247±0.013	−22% ***	0.238±0.019	−24% **
% time spent	G S P	15.4 21.5 63.1	18.7 24.9 56.4		27 19.5 53.4
Transition frequency (min−1)	C	2.25±0.26	2.63±0.17	NS	2.15±0.30	NS
	R	6.48±0.35	7.75±0.42	+20% *	6.48±0.62	NS
Transition frequency (µm-1)	C	1.74±0.16	1.48±0.14	NS	1.18±0.21	−32% *
	R	0.88±0.04	1.01±0.05	NS	0.64±0.08	−27% **
Dynamicity (µm/min)		3.0	4.2	+40%	4.5	+50%

Variables of microtubule dynamic instability were determined on living HMEC-1 cells after 1 h incubation with 100 nM native or BPB-inactivated MVL-PLA2. G: growth; S: shortening; P: pause; C: catastrophe; R: rescue; NS: non significant.

Student's t test: * p<0.05; ** p<0.01; *** p<0.001.