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. 2010 Apr 12;5(4):e10139. doi: 10.1371/journal.pone.0010139

Figure 3. Glutamate excitotoxicity decreases GAD65/67 mRNA.

Figure 3

Gene expression was analysed in cultured hippocampal neurons (7 DIV) exposed or not to 125 µM Glutamate, for 20 min, and then returned to the original culture medium for 4 h. For the reverse transcription reaction 1 µg of total RNA was used. The results were normalized with two internal control genes, GAPDH and Tubulin. Data are presented as mean±SEM of four to five independent transcription reactions, performed in independent preparations. Statistical analysis was performed using Student's t-test. *p<0.05; **p<0.01.