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. 2010 Apr 12;5(4):e10122. doi: 10.1371/journal.pone.0010122

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Azakir et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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His-myc-dysferlin purified on Ni-NTA beads was incubated with polymerized microtubules. Reactions were resolved by SDS-PAGE stained with SimplyBlue SafeStain. Arrows point to His-myc-dysferlin, tubulin, BSA, and to MAP1&MAP2 of the microtubule-associated protein fraction (MAPF), which includes MAP2A, MAP2B, MAP1 and tau. S: Soluble phase, P: Pellet. Lane 1: Microtubules alone, lane 2: Microtubules incubated with MAPF, lane 3: Microtubules incubated with BSA, lane 4: MAPF alone, lane 5: BSA alone, lane 6: Purified His-myc-dysferlin alone, lane 7: Microtubules incubated with purified His-myc-dysferlin.