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. 2010 Apr 12;5(4):e10133. doi: 10.1371/journal.pone.0010133

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Chen et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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The transcription levels of four pathway genes were detected. The levels of both mRNAs relative to the Seβ-actin mRNA level were measured with real-time PCR. The mRNA expression level in the non-injection group is designated as one. Each point represents the mean ± SEM from three independent experiments with three effective individuals in each replicate. An asterisk indicates significant differences of the mRNA levels between the dsGFP and dsSeTre-1 or dsSeTre-2 groups measured at the same time (p<0.05, T test) and double asterisks indicates very significant differences (p<0.01, T test). G-6-P-I: glucose-6-phosphate isomerase gene, UAP: UDP-N-acetylglucosamine pyrophosphorylase gene, CHSA: chitin synthase gene A, CHSB: chitin synthase gene B.