Table 3. PCR primers used in this study.
Primers | Primer sequence |
Degenerate primers | |
SeTre-1-F1 | 5′-AGYGGYTGGGAYTTCTC-3′ |
SeTre-1-F2 | 5′-TGGATYATBGAAGGTCT-3′ |
SeTre-1-R1 | 5′-GCCADGCGTTRGGGAAGTCC-3′ |
SeTre-1-R2 | 5′-CGCRTCRTAYTTCTCRAACAT-3′ |
For cDNA cloning | |
5-SeTre-1-1 | 5′-CACTATATTCGGATCGACAG-3′ |
5-SeTre-1-2 | 5′-GGAGCCAGGTTAGATGGGT-3′ |
3-SeTre-1-1 | 5′-CACACCAGATACATCATACC-3′ |
3-SeTre-1-2 | 5′-GGAATCACCGGGATGCTG-3′ |
For real-time PCR | |
QSeTre-1-F | 5′-ATTCGCCAGAAACATCACCAAC-3′ |
QSeTre-1-R | 5′-TTCCACTTATCAGCAGACCTCC-3′ |
QSeTre-2-F | 5′-GGACTCTTGGGTTGATGGTGT-3′ |
QSeTre-2-R | 5′-AGGCTTCTCAGTTCCGTGTAGG-3′ |
QActin-F | 5′-TGCGTGACATCAAGGAGAAGC-3′ |
QActin-R | 5′-CCATACCCAAGAAGGAAGGCT-3′ |
QG-6-P-I-F | |
eG-6-P-I-R | |
QUAP-F | 5′-AGCAGACGGCAGACTAACTTTC-3′ |
QUAP-R | 5′-GGACTCCTTCGTGGTCAACATAA-3′ |
QCHSA-F | 5′-TAAGGCAAAGATTCGTCACAGG-3′ |
QCHSA-R | 5′-CAGGGTCAGCAGATAGGTGTTC-3′ |
QCHSB-F | 5′-CGCTGAGTCTTGTTGGTCCTGT-3′ |
QCHSB-R | 5′-TCCACGCTACCTCTTTCCCTA-3′ |
For dsRNA synthesis | |
dsSeTre-1-F | 5′-ACCAGGAACCGTCAGTAG-3′ |
dsSeTre-1-R | 5′-GCAACCATAGCTGTCAACA-3′ |
dsSeTre-2-F | 5′-GCCAGGACAGGTTCACATC-3′ |
dsSeTre-2-R | 5′- GCTTCACCATCGGAATTAGG-3′ |
GFP-F | 5′-AAGGGCGAGGAGCTGTTCACCG-3′ |
GFP-R | 5′-CAGCAGGACCATGTGATCGCGC-3′ |
F: forward, R: reverse.