Figure 4. Inhibition of EBNA1 transcription activation function in cell-based assays.

Soluble compounds SC7, SC11, and SC19 were assayed for their ability to inhibit either EBNA1 or Zta-dependent transcription activation in transfected 293T cells. Cells were transfected with vector or EBNA1 expression plasmid and the OriP-Cp-Luciferase reporter plasmid in the presence of 5 µM SC7, SC11, SC19 or DMSO control. 100% inhibition was equivalent to basal expression levels of OriP-Cp-Luc in the absence of ectopic EBNA1 expression. In parallel experiments, the same compounds were also assayed for inhibition of Zta transcription activation of the BHLF1-Luciferase reporter plasmid. Percent inhibition of Zta is shown in grey. Percent inhibition of EBNA1 is shown in black. Error bars represent standard deviation from the mean for at least three experimental replicates.